Isolation, Identification, and Genetic Evolution Analysis of VP1 Gene of Feline Calicivirus Strain ZZ202306
This study investigated a suspected Feline calicivirus (FCV) outbreak at a veterinary facility in Zhengzhou, Henan Province, China. RT-PCR analysis confirmed the FCV presence, with subsequent CRFK cell culture propagation leading to the isolation and characterization of strain ZZ202306. Immunofluorescence and Western blot analyses validated the specificity of monoclonal antibodies targeting the FCV VP1 capsid protein. Transmission electron microscopy revealed non-enveloped virions of ~40 nm in diameter, exhibiting typical caliciviral architecture. Viral replication kinetics demonstrated exponential growth between 6 and 18 h post-inoculation, reaching a peak titer of 10<sup>7.96</sup> TCID<sub>50</sub>/0.1 mL. Genomic sequencing coupled with phylogenetic reconstruction of the <i>VP1</i> gene revealed a close genetic relation to domestic Chinese strains and international variants, while maintaining distinct evolutionary divergence from other calicivirus genera.
Authors
- Shibo Zhao (ORCID: https://orcid.org/0000-0003-1801-8156)
- Dan Su (ORCID: https://orcid.org/0000-0002-1059-5226)
- Shijun Zhang (ORCID: https://orcid.org/0000-0001-9732-5925)
- Jie Xing (ORCID: https://orcid.org/0000-0002-9732-2318)
- Sheng‐Li Ming (ORCID: https://orcid.org/0000-0002-7505-6143)
- Bei‐Bei Chu (ORCID: https://orcid.org/0000-0003-2961-4754)
- Jiang Wang (ORCID: https://orcid.org/0000-0003-3519-4987)
- Lei Zeng (ORCID: https://orcid.org/0000-0002-2470-7073)
Institutions
- Ministry of Agriculture and Rural Affairs (CN)
- Henan Agricultural University (CN)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2025-03-13
- DOI
- https://doi.org/10.3390/ijms26062565
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- Henan Provincial Science and Technology Research Project