Genome‐Wide Association Studies Reveal the Genetic Architecture of Ionomic Variation in Grains of Tartary Buckwheat
Tartary buckwheat (Fagopyrum tataricum) is esteemed as a medicinal crop due to its high nutritional and health value. However, the genetic basis for the variations in Tartary buckwheat grain ionome remains inadequately understood. Through genome-wide association studies (GWAS) on grain ionome, 52 genetic loci are identified associated with 10 elements undergoing selection. Molecular experiments have shown that the variation in FtACA13's promoter (an auto-inhibited Ca<sup>2+</sup>-ATPase) is accountable for grain sodium concentration and salt tolerance, which underwent selection during domestication. FtYPQ1 (a vacuolar amino acid transporter) exhibits zinc transport activity, enhancing tolerance to excessive zinc stress and raising zinc accumulation. Additionally, FtNHX2 (a Na<sup>+</sup>/H<sup>+</sup> exchanger) positively regulates arsenic content. Further genomic comparative analysis of "20A1" (wild accession) and "Pinku" (cultivated accession) unveiled structural variants in key genes involved in ion uptake and transport that may result in considerable changes in their functions. This research establishes the initial comprehensive grain ionome atlas in Tartary buckwheat, which will significantly aid in genetic improvement for nutrient biofortification.
Authors
- Meiliang Zhou (ORCID: https://orcid.org/0000-0001-5175-3684)
- Yaliang Shi (ORCID: https://orcid.org/0000-0002-3600-6290)
- Xiang‐Qian Liu (ORCID: https://orcid.org/0000-0002-9713-0498)
- Dili Lai
- Muriel Quinet (ORCID: https://orcid.org/0000-0001-5485-6582)
- Jiayue He (ORCID: https://orcid.org/0000-0001-5756-6345)
- Mengyu Zhao
- Sun‐Hee Woo (ORCID: https://orcid.org/0000-0003-1444-3438)
- Yuqi He (ORCID: https://orcid.org/0000-0001-9920-9653)
- Alisdair Robert Fernie (ORCID: https://orcid.org/0000-0001-9000-335X)
- Xin‐Yuan Huang
- Tong Liu (ORCID: https://orcid.org/0000-0003-1077-5101)
- Zhirong Wang (ORCID: https://orcid.org/0000-0002-3919-8304)
- Lin Hao (ORCID: https://orcid.org/0000-0002-0828-3581)
- Kaixuan Zhang (ORCID: https://orcid.org/0000-0001-9562-1675)
- Wei Li (ORCID: https://orcid.org/0000-0003-3633-1082)
- Xiangru Wang
- Xiaoyang Peng
- Guijie Lei
Institutions
- Nanjing Agricultural University (CN)
- Chungbuk National University (KR)
- Yunnan University (CN)
- Max Planck Institute of Molecular Plant Physiology (DE)
- Chinese Academy of Agricultural Sciences (CN)
- Sanya University (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Institute of Crop Sciences (CN)
- UCLouvain (BE)
Publication Details
- Journal
- Advanced Science
- Published
- 2025-03-13
- DOI
- https://doi.org/10.1002/advs.202412291
- Citations
- 2
- Primary Topic
- Seed and Plant Biochemistry
- Type
- article
- Field-Weighted Citation Impact
- 2.32
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities