Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model

Abstract—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2.