Impact of Silages Made of Lignocellulosic Biomass on Rumen Fermentation In-Vitro and Potential Mitigation of Acid Accumulation by Bentonite Supplementation

The main purpose of this experimental study was to assess the accumulation of organic acids and their mitigation in rumen cultures fermenting silages.The silages used in the rumen fermentation were composed of corn stalks, bagasse, and rice straw.The rumen fermentation was conducted in vitro using batch systems at 38.5 0.5 for 48 hours.The results showed that within 4 hours of incubation, the rumen culture fermenting bagasse silage produced 40% more VFA than other silages.Lactic acid build-up occurred exclusively in the rumen culture fermenting bagasse silage.Lactic acid production peaked after 8 hours of incubation, reaching a pH of 5.5.The addition of bentonite to the rumen culture fermenting bagasse silage reduced lactic acid concentration by 65%.Supplementing the feed with 10% bentonite can significantly increase pH from 5.5 to 6.2, potentially mitigating ruminal acidosis.