Mining of Novel Myrosinase with High Activity Based on Sequence and Structure Clustering for Efficient Preparation of Sulforaphane
Sulforaphane has garnered significant research attention owing to its potent and promising biological activities. Mining the highly active myrosinase is the key to preparing sulforaphane. In this study, a novel myrosinase, designated Semyr, was identified from <i>Serratia plymuthica</i> through sequence and structural clustering analysis. The enzyme was heterologously expressed in <i>Escherichia coli</i>, demonstrating a sinigrin hydrolysis activity of 110.48 U/mg, which constitutes the highest recombinant myrosinase activity reported to date. A reaction system was established to prepare sulforaphane. 60 U of myrosinase was added to 5 mL of substrate, yielding 15.39 mg of sulforaphane per gram of broccoli seeds after 20 min at 40 °C and pH 6.0, with a conversion rate of 96.50%. Concurrently, the highest productivity of 5.55 μmol/g·min for sulforaphane was achieved after 15 min. Thus, Semyr serves as a valuable biocatalytic tool for the efficient preparation of sulforaphane.
Authors
- Mohamed Abdel aziz Balah (ORCID: https://orcid.org/0000-0002-1581-5558)
- Changhu Xue (ORCID: https://orcid.org/0000-0002-6976-2906)
- Hamed I. Hamouda (ORCID: https://orcid.org/0000-0002-5980-0796)
- Xiangzhao Mao (ORCID: https://orcid.org/0000-0002-6315-1338)
- Hong Jiang (ORCID: https://orcid.org/0000-0003-2939-2514)
- Suxue Chen
- Jiaqi Wang
- Zhe Wang (ORCID: https://orcid.org/0000-0003-3762-3167)
- Yán Li (ORCID: https://orcid.org/0000-0002-3842-5667)
Institutions
- China Light Industry Press (China) (CN)
- Institute of Plant Protection (CN)
- Qingdao National Laboratory for Marine Science and Technology (CN)
- Desert Research Center (EG)
- Ocean University of China (CN)
- Egyptian Petroleum Research Institute (EG)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2025-03-13
- DOI
- https://doi.org/10.1021/acs.jafc.5c00978
- Citations
- 4
- Primary Topic
- Genomics, phytochemicals, and oxidative stress
- Type
- article
- Field-Weighted Citation Impact
- 3.22
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hainan Province
- Natural Science Foundation of Shandong Province
- Taishan Scholar Project of Shandong Province
- Fundamental Research Funds for the Central Universities