Pharmaceutico-analytical Study of Dhananjayadi Vati and Assessment of its Antihistaminic Activity: A Research Protocol

Introduction: Histamine is the local hormone synthesised by mast cells in the tissue and basophils in the blood. Histamine and its Receptors (H1R-H4R) have a substantial impact on the onset of many allergic diseases. Allergic diseases, including asthma, pruritus, atopic dermatitis and allergic rhinitis, result from the complex interaction of inflammatory cells like basophils, mast cells, lymphocytes and dendritic cells responding to various environmental and allergic stimuli. Need of the study: An antihistamine is a medication that works against histamines to relieve allergy symptoms. These medications help to address conditions triggered by histamine in the body. However, some non steroidal antihistaminic drugs are proven to be quick-acting but are associated with potential side-effects, such as organ damage. In Ayurveda, several medicinal preparations have been mentioned to combat the allergic condition and the Dhananjayadi tablet (Vati) might also be significantly effective in allergic conditions. Aim: Pharmaceutico-analytical evaluation of Dhananjayadi Vati and assessment of its antihistaminic activity. Materials and Methods: An in-vitro study will be conducted in the Department of Dravyaguna at Mahatma Gandhi Ayurved College Hospital Research Centre, Salod (H) Wardha, Maharashtra, India from August 2024 to March 2025. All herbal drugs will be collected, verified and primarily authenticated by the Department of Dravyaguna and will be prepared as per the references. Organoleptic parameters (touch, appearance, taste, odour) and physicochemical parameters (hardness, pH value, uniformity of weight, loss of drying at 105°C, total ash, acid-insoluble ash, water-soluble extractive values, alcohol-soluble extractive values, disintegration time, friability and High-Performance Thin-Layer Chromatography (HPTLC) of the drug) will be evaluated. The antihistaminic action of Dhananjayadi tablet (Vati) will be evaluated in-vitro while assessing the proportion of contraction of goat tracheal tissue. A paired t-test will be conducted for pre- and postintervention evaluation and a p-value <0.001 will be considered statistically significant.