Effect of Wheat Varieties and Cultivation Environments on Grain Endophytes, Microbial Communities, and Quality of Medium-High Temperature Daqu in Chinese Baijiu

Wheat grain serves as the primary raw material for producing medium-high temperature (MT)-Daqu, a fermentation starter crucial for Chinese Baijiu production, characterized by spontaneous fermentation without the inoculation of exogenous substances. However, the interactions among wheat varieties, cultivation environments, and the resulting Daqu quality remain poorly understood. This study evaluates three wheat varieties harvested from three distinct cultivation environments, examining wheat grain quality, grain-associated endophytes, and physicochemical properties and microbial communities of MT-Daqu at 0, 9, and 90 days of fermentation. The results revealed the cultivation environment had the most pronounced impact on wheat fungal endophytes. The physicochemical properties of Daqu were primarily impacted by variety, namely, the enzyme activity impacted by environmental factors. <i>Pantoea</i>, <i>Aspergillus</i>, and <i>Stephylium</i> are key microbial genera shared between wheat grains and MT-Daqu. Redundancy analysis highlighted the critical roles of moisture content, starch content, and amino acid nitrogen levels in driving microbial succession in Daqu. Mantel analysis demonstrated significant correlations between the abundance of dominant fungal endophytes in wheat grains and Daqu quality parameters, including starch content (r = 0.45; <i>p</i> < 0.01), saccharifying activity (r = 0.41), liquefying activity (r = 0.31), and esterifying activity (r = 0.30) (<i>p</i> < 0.05). Spearman correlation analysis indicated that <i>Nesterenkonia</i>, <i>Aspergillus</i>, <i>Cryptococcus</i>, <i>Dioszegia</i>, <i>Golubevia</i>, <i>Udeniomyces</i> and <i>Stemphylium</i> are the dominant wheat-derived bacterial genera associated with the abundance of microorganisms in MT-Daqu. This study elucidated the "cultivation environment-grain endophyte-Daqu microorganism" microbial transmission pathway, providing a theoretical foundation for breeding wheat varieties optimized for Daqu production and identifying suitable production regions.