Hangeshashinto Increases the Resistance of Cells Against Cisplatin Cytotoxicity by Regulating the Cell Cycle

Objectives Oral mucositis is a common complication of chemotherapy and/or radiotherapy (CRT), often leading to severe pain, difficulty in eating, and weight loss, which can disrupt cancer treatment. Hangeshashinto (HST), a traditional Kampo medicine, has been reported to suppress CRT-induced oral mucositis. This study aimed to investigate the effects of HST on chemotherapy-induced oral mucositis and uncover its protective mechanisms. Material and Method The TR146 oral mucosa cell line was used. Cell growth with HST was evaluated using the MTT assay. The expression of cell proliferation-related genes was analyzed by quantitative real-time PCR. The effects of HST combined with cisplatin were assessed by cell cycle analysis. Results The MTT assay showed that HST reduced cell proliferation. PCR revealed that HST lowered the expression of CCND1 and CCND3 . Cell cycle analysis indicated that HST promoted G0/G1 and G2/M phase arrest, reducing cell death and apoptosis after cisplatin treatment. Conclusions HST enhances resistance to cisplatin-induced cytotoxicity by regulating the cell cycle, suggesting its potential as a protective agent against chemotherapy-induced oral mucositis.