“Unveiling the Anti-Diabetic Potential of Lindernia ciliata by using in-vitro methods: Phytochemical Insights and Mechanistic Pathways"

Objective: To evaluate the phytochemical screening and anti-diabetic activity of different fractions obtained from the hydroalcoholic extract of Lindernia ciliata (Colsm.) Pennell. Methodology: The hydroalcoholic extract of L. ciliata, a member of the Linderniaceae family, was fractionated using petroleum ether, chloroform, methanol, and water. These fractions were analyzed for secondary metabolites through phytochemical screening, and the total phenolic and flavonoid contents were determined. The anti-diabetic activity of the fractions was assessed using multiple assays, including glucose absorption, glucose uptake, insulin secretion by RIN-5F cells, α-amylase inhibition, and DPP-4 inhibition.