Komagataella phaffii: A versatile platform for the production of value-added chemicals

The development of green biomanufacturing technologies centered around advanced microbial cells has emerged as a hot research field in synthetic biology. As known, Komagataella phaffii is an unconventional yeast with unique biological characteristics, which is generally regarded as safe (GRAS). Owing to its wide substrate spectrum, K. phaffii has been widely genetically modified for valuable chemicals such as organic acids, fatty acids and some nature products. Actually, K. phaffii is very well known for the protein expression, and few reviews have systemically addressed the value-added chemicals by K. phaffii . Accordingly, this review will introduce the most advanced development of genetic editing tools of K. phaffii . Recent progresses and bottlenecks in the production of high-value chemicals using K. phaffii will also be summarized. Finally, future perspectives for the utilization of K. phaffii as a chassis cell used for high-value products synthesis will be discussed. • Systematically introduce the latest developments in gene editing tools for K. phaffii . • Comprehensive summary of high-value chemicals synthesized by K. phaffii . • Elaborate on the metabolic regulation strategies of K. phaffii . • In-depth discussion on the challenges and future prospects of using K. phaffii as a chassis cell.