The GP2a 91/97/98 amino acid substitutions play critical roles in determining PRRSV tropism and infectivity but do not affect immune responses
Prevalent PRRSV isolates present different cell tropisms <i>in vitro</i>. Clarifying the exact determinant of PRRSV tropism is crucial for PRRSV isolation and vaccine development. By constructing chimeric viruses based on four representative PRRSV infectious clones, we identified for the first time that the 91/97/98 amino acid substitutions in GP2a play critical but distinct roles in determining Marc-145 cell tropism for different PRRSV strains. The GP2a 91/97/98 amino acid substitutions also affect PRRSV infectivity in PAMs and piglets but do not influence immune responses. This study not only deciphers an exact determinant of PRRSV tropism and infectivity but also has guiding significance for PRRS vaccine development.
Authors
- Nanhua Chen (ORCID: https://orcid.org/0000-0002-1909-8760)
- Wenhao Qi (ORCID: https://orcid.org/0000-0001-9106-7734)
- François Meurens (ORCID: https://orcid.org/0000-0002-0353-4871)
- Shubin Li
- Yuejia Qiu
- Jianzhong Zhu (ORCID: https://orcid.org/0000-0002-7082-1993)
- Shaobin Shang (ORCID: https://orcid.org/0000-0003-4407-1911)
- Zhe Sun (ORCID: https://orcid.org/0000-0001-6194-4054)
- Lin Hong (ORCID: https://orcid.org/0000-0002-1288-7150)
- Ming Qiu (ORCID: https://orcid.org/0000-0001-7268-3195)
- Shuai Li (ORCID: https://orcid.org/0000-0002-2935-4473)
- Xiuling Yu
- Shuai Yang
- Kegong Tian (ORCID: https://orcid.org/0000-0002-1420-6347)
- Meng Cui
Institutions
- Center for Veterinary Medicine (US)
- Université de Montréal (CA)
- Luoyang Institute of Science and Technology (CN)
- Yangzhou University (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2025-03-12
- DOI
- https://doi.org/10.1128/jvi.00048-25
- Citations
- 8
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 9.55
Funders
- National Natural Science Foundation of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Higher Education Discipline Innovation Project