Study of Antibiotic Sensitivity Pattern of Aerobic Gram-positive Organism Isolated from Pus Samples in Tertiary Care Hospital

A BSTRACT Introduction: Localized inflammation brought on by pyogenic bacteria, which can lead to a buildup of infectious agents and dead leukocytes, is what sets apart pyogenic infections. In general, this infection presents with pus formation during infection at the site of inflammation, with a collection of thick, whitish, or yellowish liquid is termed to as pus. A break in the skin can allow surface bacteria to enter and begin multiplying locally. A pyogenic infection, which inhibits wound healing and can lead to consequences, including wound dehiscence or disintegration, is caused by the buildup of these cells, which results in pus. Aim: The bacteriological profile and antibiogram of the organisms isolated from pus in a tertiary care hospital setting are to be determined. Methodology: This study was a prospective cross-sectional study conducted in the Department of Microbiology at Jawaharlal Nehru Medical College associated with Acharya Binova Vava Rural Hospital (ABVRH), Sawangi, Meghe; Wardha. This study was carried out from August 2019 to August 2021. A Total of 139 samples were collected from various departments of ABVRH. The collected samples were processed with microscopic examination, culture, characterization, and identifications of the isolated organism with the help of different identification tests with the standard method and antibiotic sensitivity were also performed using Kirby–Bauer disc diffusion method and result were recorded using the standard Clinical and Laboratory Standards Institute guideline. Results: Among 139 pus samples, 97 (69.78%) were from male patients, and 42 (30.22%) were female patients. The highest percentage of pus samples were from the orthopedic department and the surgery department. Out of the total pus samples, 50 samples showed Gram-positive bacteria. Among which, Staphylococcus aureus (82%) was predominant, followed by Enterococcus species (10%) and coagulase negative Staphylococci (8%). Out of 41 isolates of S . aureus , 23 (56.10%) were methicillin-resistant S. aureus (MRSA) and 18 (48.90%) were methicillin susceptible S . aureus (MSSA). In this study, MRSA was more prevalence to MSSA. Conclusion: Vancomycin, linezolid, and tetracycline were found to be effective against S . aureus strains that were identified as MRSA. For effective wound infection management, the causal agents can be identified and the sensitivity to antibiotics evaluated by carefully using standard microbiological procedures.