Development of Fluorescence-Based Method for Dopamine Determination Using o-Phthaldialdehyde and 3-Mercaptopropyltriethoxysilane
Nanomaterials and sensors play an important role in modern technologies, including medical diagnostics and biochemical research. This work presents the possibility of using o-Phthaldialdehyde (OPA) in combination with 3-mercaptopropyltriethoxysilane (MPTES) to develop a dopamine-responsive sensor. During the experiment, these materials were used at different pH and ratios to determine the optimal parameters for obtaining high fluorescence intensity of the reaction product. The data obtained demonstrate a linear relationship between the fluorescence response (λ<sub>ex</sub>/λ<sub>em</sub> = 340/460 nm) of OPA/MPTES and dopamine concentration in the range of 0.1-3.0 µM at a pH of 8, and the detection limit was 8.7 nM. The obtained results confirm the potential of OPA/MPTES as a sensing component for the detection of dopamine.
Authors
- Miloš Krstić (ORCID: https://orcid.org/0000-0003-0267-0203)
- Valeriia V. Sliesarenko (ORCID: https://orcid.org/0000-0003-0287-656X)
- Aleksandra Lobnik
- Urban Bren
Institutions
- University of Primorska (SI)
- University of Maribor (SI)
- Institute for Environmental Protection and Sensors (SI)
Publication Details
- Journal
- Sensors
- Published
- 2025-03-11
- DOI
- https://doi.org/10.3390/s25061729
- Citations
- 4
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 2.92
Funders
- Javna Agencija za Raziskovalno Dejavnost RS