Comparative Chemoprofile Variation Study of Bhumyamalaki (Phyllanthus fraternus Webst) Collected from Different Bhoomi Desha of Karnataka by Using RP-UPLC Method

As per the survey of WHO, 88% of all countries are estimated to use traditional medicines. Ayurveda is bestowed science of life which is mainly based on herbs. It is a treasure of amazing herbal medicine but still many important medicinal plants which are used in treatment are need to be standardized. The drug Tamalaki mentioned in Swasahara and Kasahara dashemani of Charaka Samhita which is popularly known as Bhumyamalaki (Phyllanthus fraternus webst). Phyllanthus fraternus Webst (syn. P.niruri auct. Pl.non Linn) is one of the species belongs to Euphorbiaceae family is considered as Bhumyamalaki, which is commonly found as a weed in Central and southern India. At present, many species of Bhumyamalaki are found in natural habitat. In Ayurveda, seers have given importance to Bhoomi Desha i.e the natural habitat of dravyas is important in Dravya collection wherein they have emphasized to collect medicinal plants from suitable desha. Many literatures survey revealed the presence of various phyto-constituents in Phyllanthus fraternus such as Phyllanthin, Hypophyllanthin, Nirphyllin, Phillnirurin, Niranthin, Niruriside, Securinine, Limonene, 4-Methoxy- securinine, 4-Methoxy-norsecurinine, Niruretin, Phyllanthol, Phyllanthenol, Phyllanthenone, Lintetralin, Astragalin, Cymene, Niruodine and Phyllanthamide. The present work aims to see the possible changes in Phyto constituents seen in a same species of Bhumyamalaki i.e. Phyllanthus fraternus Webst which is grown in different Desha like Jangaladesha, Sadharanadesha and Anoopadesha, Quantification of Phytochemicals are done by using TLC and RP-UPLC. Result : The Percentage of Phytoconstituents Phyllanthin and Hypophyllanthin was found to be more in anupa desha sample when compared to other two deshas sample.