A rhizobacterium-secreted protein induces lateral root development through the IAA34-PUCHI pathway
Lateral roots (LRs) can continuously forage water and nutrients from soil. In Arabidopsis thaliana, LR development depends on a canonical auxin signaling pathway involving the core transcription factors INDOLE-3-ACETIC ACIDs (IAAs) and AUXIN RESPONSE FACTORs (ARFs). In this study, we identified a protein, bacillolysin, secreted by the beneficial rhizobacterium Bacillus velezensis SQR9, that is able to stimulate LR formation of Arabidopsis. The receptor protein kinase C-TERMINALLY ENCODED PEPTIDE RECEPTOR2 (CEPR2) interacts with bacillolysin and plays a critical role in LR development. In the bacillolysin-regulated signaling pathway, the transcriptional repressor IAA34 interacts with PUCHI to activate downstream LATERAL ORGAN BOUNDARIES-DOMAIN33 (LBD33) expression, consequently inducing LR development. This study reveals interkingdom communication via a protein that mediates a novel pathway to induce LR development.
Authors
- Jintao Xiao
- Zhihui Xu (ORCID: https://orcid.org/0000-0002-3987-8836)
- Youzhi Miao (ORCID: https://orcid.org/0000-0002-0645-0745)
- Qirong Shen (ORCID: https://orcid.org/0000-0002-5662-9620)
- Weibing Xun (ORCID: https://orcid.org/0000-0003-0068-8514)
- Wei Xuan (ORCID: https://orcid.org/0000-0002-4859-2637)
- Yunpeng Liu (ORCID: https://orcid.org/0000-0002-3016-7165)
- Yu Chen (ORCID: https://orcid.org/0000-0001-8484-040X)
- Ruifu Zhang (ORCID: https://orcid.org/0000-0002-3334-4286)
- Nan Zhang (ORCID: https://orcid.org/0009-0000-0135-6437)
- Yuanming Xie (ORCID: https://orcid.org/0009-0007-5798-7407)
- Yansong Fu (ORCID: https://orcid.org/0009-0000-5654-4304)
Institutions
- Nanjing Agricultural University (CN)
- Institute of Agricultural Resources and Regional Planning (CN)
- Chinese Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1016/j.celrep.2025.115414
- Citations
- 8
- Primary Topic
- Legume Nitrogen Fixing Symbiosis
- Type
- article
- Field-Weighted Citation Impact
- 14.00
Funders
- Chinese Academy of Agricultural Sciences
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities
- Agricultural Science and Technology Innovation Program
- Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences