MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: application in viral infection diagnostics
An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor's functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identification via genetic code recognition. CNDsTy allow the electrochemical detection based on their different affinity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identification. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples.
Authors
- Marina Garrido (ORCID: https://orcid.org/0000-0002-4263-0989)
- Tania García‐Mendiola (ORCID: https://orcid.org/0000-0002-7634-5844)
- Estefanía Enebral-Romero (ORCID: https://orcid.org/0000-0001-9419-371X)
- Cristina Navío (ORCID: https://orcid.org/0000-0002-1443-6396)
- Emiliano Martínez‐Periñán (ORCID: https://orcid.org/0000-0003-3122-3381)
- Mónica Luna (ORCID: https://orcid.org/0000-0002-7104-0726)
- Encarnación Lorenzo (ORCID: https://orcid.org/0000-0001-8432-9652)
- Emilio M. Pérez (ORCID: https://orcid.org/0000-0002-8739-2777)
- David López-Diego (ORCID: https://orcid.org/0009-0003-0396-1077)
Institutions
- IMDEA Nanoscience (ES)
- Institute of Micro and Nanotechnology (ES)
- Universidad Autónoma de Madrid (ES)
Publication Details
- Journal
- Microchimica Acta
- Published
- 2025-03-11
- DOI
- https://doi.org/10.1007/s00604-025-07084-2
- Citations
- 1
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Comunidad de Madrid
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Ministerio de Economía y Competitividad
- Universidad Autónoma de Madrid
- Ministerio de Ciencia e Innovación
- Agencia Estatal de Investigación