Exploring metallic nanoparticles for enhanced multiplexed SERS for diagnostics.

Abstract When it comes to diagnostics and disease management, while physicians focus on the prognosis and mortality caused by viral diseases, it is necessary to be thorough about metabolic chronic illnesses that could cause complications. Owing to its remarkable sensitivity and capability for multiplexing, Surface-Enhanced Raman Spectroscopy (SERS) emerges as a potent analytical approach with substantial promise in the realms of bioanalysis and diagnostics. This work focuses on exploring metallic nanoparticles that can be used for SERS-based pathogen and metabolic disorder-biomarkers detection for rapid viral infection and chronic disease diagnosis using SARS-CoV-2 or HIV pseudo-virus and a diabetes biomarker, glucose. Herein, metallic nanoparticles (NPs) such as gold (Au) and silver (Ag) nanoparticles, were assessed for their sensitivity in detecting both disease-biomarkers in a buffer containing HIV pseudo-virus and glucose.