Enrichment of Microbial DNA in Plasma to Improve Pathogen Detection in Sepsis: A Pilot Study
Our results demonstrate that combining ssDNA library preparation together with fragment size selection improves mDNA yield, potentially reducing sequencing requirements. However, at the genus level, this combination also increases background noise, which limits sensitivity for pathogen detection.
Authors
- Eddie G. Dominguez (ORCID: https://orcid.org/0000-0002-6810-8670)
- Michelle D. Stephens (ORCID: https://orcid.org/0000-0002-8825-7999)
- Mary Junak (ORCID: https://orcid.org/0000-0001-9689-0631)
- Mehreen Kisat
- Caitlin S. Pepperell (ORCID: https://orcid.org/0000-0002-6324-1333)
- Megan L. Nedden (ORCID: https://orcid.org/0000-0002-7180-6453)
- Bradon R. McDonald (ORCID: https://orcid.org/0000-0002-6150-8735)
- Haikun Zhang (ORCID: https://orcid.org/0000-0002-3310-7029)
- Skip Thompson
- Elise C. Dietmann (ORCID: https://orcid.org/0009-0001-0771-7501)
- Nicole Byington
Institutions
- University of Wisconsin–Madison (US)
Publication Details
- Journal
- Clinical Chemistry
- Published
- 2025-03-11
- DOI
- https://doi.org/10.1093/clinchem/hvaf011
- Citations
- 4
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 2.36
Funders
- Norges Idrettshøgskole