Use of arduino‐based potentiometric sensors to measure changes in leaf apoplastic pH in common bean ( <i>Phaseolus vulgaris</i> L.)

Abstract Following an abiotic or biotic stress, the pH of the extracellular space or the apoplast of a plant can change dramatically, such as wounding that causes an increase in pH or alkalinization. In this proof‐of‐concept‐study, a newly developed carbon‐based bendable potentiometric sensor was tested for the first time in vivo on common bean ( Phaseolus vulgaris L.). The sensor was able to detect the same magnitude and direction of pH change after wounding as the conventional infiltration‐centrifugation method in five out of eight tested common bean cultivars. This highly scalable, non‐destructive, and cheap carbon‐based sensor could be used in the future within plant breeding to screen large populations of plants for responses to plant stresses.

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Publication Details

Journal
The Plant Phenome Journal
Published
2025-03-08
DOI
https://doi.org/10.1002/ppj2.70020
Citations
1
Primary Topic
Analytical Chemistry and Sensors
Type
article
Field-Weighted Citation Impact
0.74
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article

Use of arduino‐based potentiometric sensors to measure changes in leaf apoplastic pH in common bean ( Phaseolus vulgaris L.)

Jennifer Lin, Valerio Hoyos‐Villegas, Robert McGee, Ravinder Dahiya
1 citations
The Plant Phenome Journal
Analytical Chemistry and Sensors
0.74
article

Use of arduino‐based potentiometric sensors to measure changes in leaf apoplastic pH in common bean ( Phaseolus vulgaris L.)

Jennifer Lin, Valerio Hoyos‐Villegas, Robert McGee, Ravinder Dahiya
article en
1 citations

Abstract

Abstract Following an abiotic or biotic stress, the pH of the extracellular space or the apoplast of a plant can change dramatically, such as wounding that causes an increase in pH or alkalinization. In this proof‐of‐concept‐study, a newly developed carbon‐based bendable potentiometric sensor was tested for the first time in vivo on common bean ( Phaseolus vulgaris L.). The sensor was able to detect the same magnitude and direction of pH change after wounding as the conventional infiltration‐centrifugation method in five out of eight tested common bean cultivars. This highly scalable, non‐destructive, and cheap carbon‐based sensor could be used in the future within plant breeding to screen large populations of plants for responses to plant stresses.

The Plant Phenome JournalVol. 8(1)
Northeastern University (US), McGill University (CA)
Openalex Percentile: Top 37%
Analytical Chemistry and Sensors
0.74
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