Simplifying the protocol for low-pollution-risk, efficient mouse myoblast isolation and differentiation
Myoblasts are the primary effector cells that play crucial roles in myogenesis and muscle regeneration following injury. However, isolating purified primary myoblasts from murine skeletal muscle poses challenges for junior researchers. Here, we present a simplified, low-risk, and optimized protocol for the extraction and enrichment of these myogenic progenitor cells. Additionally, we demonstrate that, compared to F10 (Ham's F-10)-based medium, DMEM (Dulbecco's Modified Eagle's Medium)-based differentiation medium provides a more conducive environment for myoblasts differentiation. This enhancement improves the efficiency of myofiber formation and the expression of myogenic markers.
Authors
- Jia-Dong Zhang (ORCID: https://orcid.org/0000-0001-6378-5894)
- Qí Zhāng (ORCID: https://orcid.org/0000-0001-6300-6491)
- Zhen‐Dong Xiao (ORCID: https://orcid.org/0000-0003-1630-612X)
- Shu‐Juan Xie (ORCID: https://orcid.org/0000-0002-0867-7273)
- Li‐Ting Diao (ORCID: https://orcid.org/0009-0002-5871-5706)
- Yachao Ren (ORCID: https://orcid.org/0000-0003-4232-6466)
- Ya‐Rui Hou (ORCID: https://orcid.org/0000-0001-7275-8994)
- Wanxin Chen (ORCID: https://orcid.org/0009-0001-7189-7949)
- Yi Luo (ORCID: https://orcid.org/0000-0002-5930-7379)
- Xiaochuan Zhao (ORCID: https://orcid.org/0000-0001-5840-3601)
- Weicai Chen (ORCID: https://orcid.org/0000-0003-0609-5568)
Institutions
- Sun Yat-sen University (CN)
- Third Affiliated Hospital of Sun Yat-sen University (CN)
Publication Details
- Journal
- Advanced Biotechnology
- Published
- 2025-03-11
- DOI
- https://doi.org/10.1007/s44307-025-00060-0
- Citations
- 2
- Primary Topic
- Muscle Physiology and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.61