Genetic structure of purebreed Belgian Blue, Charolaiis and Aberdeen Angus breeds by genes associated with quality indicators of meat

Purpose : molecular genetic testing of purebred bulls of the Belgian blue, Charolais and Aberdeen Angus breeds and determination of the frequency of occurrence of genotypes and alleles by the genes of calpain, myostatin and thyroglobulin. Materials and methods . For the experiment, biological material (sperm) of purebred bulls of the blue Belgian, Charolais and Aberdeen Angus breeds was used. DNA genotyping of bulls by the genes of calpain (CAPN1), myostatin (MSDN) and thyroglobulin (TG5) was performed using the polymerase chain reaction (PCR) method and polymorphism of the lengths of the extraction fragments (PDRF). Nuclear DNA was isolated by the perchlorate method. The solution for DNA isolation was prepared according to T.Maniatis et al.[11], and solutions manufactured by ODO "Primtech" Republic of Belarus were used for amplification and restriction. Results . Having studied the genetic structure of purebred bulls of the blue Belgian, Charolais and Aberdeen Angus breeds, it was found that animals of all breeds are monomorphic to MSTN (100 %) according to the MSTN gene. According to the calpain gene (CAPN1), polymorphism was established in blue Belgian bulls and homozygous CAPN1GG genotype (50 %) and heterozygous CAPN1AG (50 %) were identified, homozygous CAPN1GG genotype (25 %) and heterozygous CAPN1AG (75 %) were identified in individuals of the Aberdeen Angus breed. According to the TG5 gene, the producing bulls of the blue Belgian breed are monomorphic TGCC (100 %). The bulls of the Charolais and Aberdeen Angus breeds have a thyroglobulin gene polymorphism: the Charolais breed has homozygous TG5CC (50 %) and heterozygous TG5TC (50 %), and the Aberdeen Angus bulls have heterozygous TG5TC (50 %) and two homozygous TG5TT (25 %) and TG5CC (25 %). Conclusions . The genetic structure of purebred blue Belgian, Charolais and Aberdeen Angus bulls based on the genes of calpain, myostatin and thyroglobulin. The genetic structure of purebred stud bulls of the Blue Belgian, Charolais and Aberdeen Angus breeds indicates that animals of all breeds are monomorphic according to the MSTN gene. In bulls of the blue Belgian breed, polymorphism was established and the homozygous genotype CAPN1GG (50%) and heterozygous CAPN1AG (50 %) were identified, and in individuals of the Aberdeen Angus breed, the homozygous genotype CAPN1GG (25 %) and heterozygous CAPN1AG (75 %) were identified. In Charolais breeding bulls, polymorphism was established for the TG5 gene and the homozygous genotype TG5CC (50%) and heterozygous genotype TG5TC (50 %) were identified.