Parallelized Droplet Vitrification for Single-Run Vitrification of Hepatocytes from an Entire Rat Liver

Drug discovery pipelines rely on the availability of isolated primary hepatocytes for investigating potential hepatotoxicity prior to clinical application. These hepatocytes are isolated from livers rejected for transplantation and subsequently cryopreserved for later usage. The gold standard cryopreservation technique, slow-freezing, is a labor-intensive process with significant poststorage viability loss. In this work, we introduce parallelized droplet vitrification, a technique for high-volumetric, rapid vitrification of suspended cells. We show the utility of this technique through the single-run vitrification of the whole rat liver hepatocyte yield, resulting in the vitrification of 250 million cells in 40 mL of a vitrification solution at 10 mL/min. Additionally, we showed that these implementations maintained improved postpreservation outcomes in primary rat hepatocytes.

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Publication Details

Journal
ACS Applied Materials & Interfaces
Published
2025-03-11
DOI
https://doi.org/10.1021/acsami.4c19419
Primary Topic
Pancreatic function and diabetes
Type
article
Field-Weighted Citation Impact
0.00

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article

Parallelized Droplet Vitrification for Single-Run Vitrification of Hepatocytes from an Entire Rat Liver

Korkut Uygun, Alexandre G. Lellouch, Mehmet Toner, Rebecca D. Sandlin et al.
ACS Applied Materials & Interfaces
Pancreatic function and diabetes
article

Parallelized Droplet Vitrification for Single-Run Vitrification of Hepatocytes from an Entire Rat Liver

Korkut Uygun, Alexandre G. Lellouch, Mehmet Toner, Rebecca D. Sandlin, McLean S. Taggart, Loïc Van Dieren, Antonia T. Dinicu, Alexandra Tchir, Huyun Chen, Christopher Taveras, Madeeha Hassan
article en

Abstract

Drug discovery pipelines rely on the availability of isolated primary hepatocytes for investigating potential hepatotoxicity prior to clinical application. These hepatocytes are isolated from livers rejected for transplantation and subsequently cryopreserved for later usage. The gold standard cryopreservation technique, slow-freezing, is a labor-intensive process with significant poststorage viability loss. In this work, we introduce parallelized droplet vitrification, a technique for high-volumetric, rapid vitrification of suspended cells. We show the utility of this technique through the single-run vitrification of the whole rat liver hepatocyte yield, resulting in the vitrification of 250 million cells in 40 mL of a vitrification solution at 10 mL/min. Additionally, we showed that these implementations maintained improved postpreservation outcomes in primary rat hepatocytes.

ACS Applied Materials & InterfacesVol. 17(11)
Harvard University (US), Inserm (FR), Massachusetts General Hospital (US), Shriners Hospitals for Children - Boston (US), Laboratoire d'Innovation Thérapeutique (FR), Center For Reconstructive Urethral Surgery (IT), Innovations Thérapeutiques en Hémostase (FR), Massachusetts Institute of Technology (US)
National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Biomedical Imaging and Bioengineering, Division of Engineering Education and Centers
Zero hunger
Openalex Percentile: Top 92%
Pancreatic function and diabetes
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