Endocannabinoid system upregulates the enrichment and differentiation of human iPSC- derived spermatogonial stem cells via CB2R agonism
We demonstrated here for the first time that stimulation of CB2R results in an increased yield of hiPSCs and hiPSC-derived hSSCs. CB65 is a potent CB2R agonist that can be used to increase the yield of hiPSC-derived hSSCs offering an alternative source of autologous male germ cells for patients with MFI. Increasing the male germ/stem cell pool by CB65 supplementation could be part of the ART-associated protocols in MFI patients with complete germ cell aplasia.
Authors
- Özgür Doğuş Erol (ORCID: https://orcid.org/0000-0001-9301-5401)
- Selin Önen (ORCID: https://orcid.org/0000-0002-3255-3035)
- Emirhan Nemutlu (ORCID: https://orcid.org/0000-0002-7337-6215)
- Fatima Aerts‐Kaya (ORCID: https://orcid.org/0000-0002-9583-8572)
- Petek Korkusuz (ORCID: https://orcid.org/0000-0002-7553-3915)
- Tuba Reçber (ORCID: https://orcid.org/0000-0001-8257-7628)
- Merve Gizer (ORCID: https://orcid.org/0000-0003-1911-2363)
Institutions
- Middle East Technical University (TR)
- Hacettepe University (TR)
Publication Details
- Journal
- Biological Research
- Published
- 2025-03-12
- DOI
- https://doi.org/10.1186/s40659-025-00596-4
- Citations
- 1
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.81