A Novel Trypsin Inhibitor With Immunomodulatory Activity From the Leaves of <i>Cissus verticillata</i> subsp. <i>verticillata</i> (L.) Nicolson & C.E. Jarvis
In this study, we purified and characterized a trypsin inhibitor (CvLTI) from the leaves of Cissus verticillata subsp. verticillata. CvLTI was isolated using a trypsin-agarose column and evaluated for stability towards heating (30°C- 100°C) and incubation at different pH values (2.0-10.0). Furthermore, CvLTI was investigated for antibacterial activity against phytopathogens, hemolytic activity as well as cytotoxicity and immunomodulatory effects on peripheral blood mononuclear cells (PBMCs). CvLTI (14.3 kDa, pI 5.3) showed sequence similarities with an endochitinase and showed a trypsin inhibitory activity thermostable and higher at acidic pH. The inhibitor exerted bacteriostatic effects on Pectobacterium isolates (minimum inhibitory concentration: 462 µg/mL). CvLTI showed hemolytic activity with an EC<sub>50</sub> of 33 µg/mL. CvLTI (12.5 and 25.0 µg/mL) induced the release of cytokines interleukin (IL)-4, IL-6, IL-10 and tumor necrosis factor-α by PBMCs. These data will drive further studies on the toxicity, antitumor, and anti-inflammatory potentials of safe doses of CvLTI.
Authors
- Georon Ferreira de Sousa (ORCID: https://orcid.org/0000-0001-8780-202X)
- Roberto Araújo Sá (ORCID: https://orcid.org/0000-0003-1895-9175)
- Robson Raion de Vasconcelos Alves (ORCID: https://orcid.org/0000-0002-7539-0491)
- Cristiane Moutinho Lagos de Melo (ORCID: https://orcid.org/0000-0002-8831-0163)
- Ana Rosa Peixoto (ORCID: https://orcid.org/0000-0002-8867-9497)
- Maria Tereza dos Santos Correia (ORCID: https://orcid.org/0000-0003-4920-9975)
- Russolina B. Zingali (ORCID: https://orcid.org/0000-0003-3156-6923)
- Pollyanna Michelle da Silva
- Abdênego Rodrigues da Silva (ORCID: https://orcid.org/0000-0001-7327-828X)
- Thâmarah de Albuquerque Lima (ORCID: https://orcid.org/0000-0002-7850-5979)
- Thiago Henrique Napoleão (ORCID: https://orcid.org/0000-0002-0065-2602)
- Patrícia Maria Guedes Paiva (ORCID: https://orcid.org/0000-0003-3467-708X)
- Lucas da Cruz Farias (ORCID: https://orcid.org/0000-0001-8867-2570)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Universidade do Estado da Bahia (BR)
- Universidade Federal de Pernambuco (BR)
Publication Details
- Journal
- Chemistry & Biodiversity
- Published
- 2025-03-11
- DOI
- https://doi.org/10.1002/cbdv.202403287
- Primary Topic
- Biochemical and Structural Characterization
- Type
- article
- Field-Weighted Citation Impact
- 0.00