Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry
Cytometry by time of flight (CyTOF) is a flow cytometry-based technique using metal-tagged antibodies, allowing immunophenotyping. Here, we present a protocol for phenotyping mouse myeloid and lymphoid cells isolated from lung, spleen, and intraperitoneal lavages in healthy or pathologic conditions. We describe steps for antibody labeling and titration, tissue dissociations, and staining. We then detail procedures to compare staining obtained from fresh or cryopreserved tissues. For complete details on the use and execution of this protocol, please refer to Morin et al.<sup>1</sup>.
Authors
- Patrice Hémon (ORCID: https://orcid.org/0000-0002-2244-5321)
- Sarah Dion (ORCID: https://orcid.org/0000-0002-7347-7462)
- Simon Le Gallou (ORCID: https://orcid.org/0009-0003-4758-2423)
- Brice Autier (ORCID: https://orcid.org/0000-0001-6945-4359)
- Valérie Lecureur (ORCID: https://orcid.org/0000-0002-8606-4755)
- Laura Morin
- Mikaël Roussel (ORCID: https://orcid.org/0000-0002-9741-0668)
Institutions
- Inserm (FR)
- Université de Bretagne Occidentale (FR)
- Université Rennes 2 (FR)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- Laboratoire des Sciences et Techniques de l’Information de la Communication et de la Connaissance (FR)
- Centre Hospitalier Universitaire de Rennes (FR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1016/j.xpro.2025.103684
- Citations
- 2
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 1.61