Seed Fatty Acids Modify Oviposition of Tenebrio molitor (Coleoptera: Tenebrionidae)

Plant-insect interactions mediated by chemical compounds are well documented in roots and above-ground plant parts except seeds. The latter chemoecological interactions remain poorly studied. The chemical composition of seeds, including attractive, repellent, or inhibitory compounds, likely influences oviposition behavior, yet specific studies on this subject are scarce. This study evaluated the oviposition behavior of the yellow mealworm (<i>Tenebrio molitor</i> L. (Coleoptera, Tenebrionidae)) on substrates of common oat (<i>Avena sativa</i> L. (Poales: Poaceae)), common wheat (<i>Triticum aestivum</i> L. (Poales: Poaceae)), rapeseed (<i>Brassica napus</i> L. (Brassicales: Brassicaceae)), and pure sand. Females laid the most eggs on oat and wheat substrates, while oviposition on sand was reduced by 22%. The chemoreceptors located in the antennae of <i>T. molitor</i> were found not to influence oviposition. Hexane extracts of oat flour were found to contain oviposition-inhibiting compounds, identified as fatty acids. Behavioral tests showed that oleic, palmitic, linoleic, and stearic acids inhibited oviposition at concentrations ranging from 5% to 0.5%. A lower concentration (0.05%) did not have this effect. Additionally, linoleic, palmitic, and oleic acids exhibited repellent properties, whereas stearic acid did not. These findings provide valuable insights into optimizing substrate composition to enhance <i>T. molitor</i> reproduction. This has applications for small-scale laboratory research and large-scale industrial production, supporting the use of <i>T. molitor</i> as an alternative protein source for feed and food.