Effectiveness of antibiofilm Aspergillus niger cell-free supernatant against Pseudomonas aeruginosa biofilm

Abstract. Cahyakirana TD, Susilowati A, Pangastuti A. 2024. Effectiveness of antibiofilm Aspergillus niger cell-free supernatant against Pseudomonas aeruginosa biofilm. Asian J Trop Biotechnol 21: 89-95. Pseudomonas aeruginosa is an opportunistic gram-negative pathogenic-bacteria that can cause nosocomial infections. Pseudomonas aeruginosa has several virulence factors that can cause infection, including its ability to form biofilms. Biofilm-forming bacteria can limit the penetration of antibiotics toward the microbes. These limitations make it challenging to treat biofilm-producing bacterial infections. Enzymes produced by Aspergillus niger with anti-biofilm activity include lactonase, ?-glucosidase, acylase, and oxidoreductase. This study aimed to test the effectiveness of cell-free supernatant (CFS) of A. niger against P. aeruginosa biofilms. The antibiofilm activity test consisted of a biofilm growth inhibition test and P. aeruginosa biofilm destruction test. The research method used was the microtiter plate biofilm assay. The results showed that CFS of A. niger had the ability to inhibit the growth and destruction of P. aeruginosa biofilms. In the biofilm growth inhibition test and biofilm destruction test, the minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) were 56 and 55.7% at 50% concentration, respectively. It was concluded that A. niger CFS had antibiofilm activity against P. aeruginosa biofilms.