Development and applications of paper-based point-of-care testing platforms for electrochemical blood glucose detection

Purpose This study aims to comprehensively analyze the current developments and applications of paper-based electrochemical platforms for blood glucose detection, focusing on their potential to revolutionize point-of-care testing through cost-effective and accessible diagnostic solutions. Design/methodology/approach The review systematically examines fundamental principles of paper-based platforms, including substrate properties, fluid transport mechanisms and electrochemical detection methods. It critically evaluates recent technological advances in materials science, fabrication techniques and signal amplification strategies while analyzing various case studies demonstrating successful implementations. Findings Recent innovations in paper-based glucose sensors have achieved remarkable performance metrics, with detection limits reaching sub-millimolar ranges and response times within seconds. The integration of nanomaterials, particularly graphene-based composites and carbon nanotubes, has significantly enhanced sensor sensitivity and stability. Advanced enzyme immobilization techniques using layer-by-layer assembly have demonstrated sustained activity for up to 10 weeks, while novel signal amplification strategies incorporating bimetallic nanoparticles have pushed detection limits into the sub-picogram range. Originality/value This review uniquely synthesizes the latest developments in paper-based electrochemical glucose sensing, providing critical insights into the synergistic integration of advanced materials, fabrication methods and detection strategies. It offers valuable perspectives on overcoming current technical challenges and highlights emerging opportunities in smart device integration and artificial intelligence applications, serving as a comprehensive resource for researchers and practitioners in the field of point-of-care diagnostics.