Effects of Vitamin C and Jamblang Leaf Extract (Syzygium cumini) on Catalase Enzyme Activity in Lead Acetate-Induced Rats

Lead is a toxic heavy metal that causes oxidative stress and suppresses endogenous antioxidants, such as the catalase enzyme. Vitamin C and jamblang leaves are exogenous antioxidants to inhibit oxidative stress. This research aimed to determine the effect of jamblang leaf extract and vitamin C on the catalase enzyme activity of mice that had been induced by acetate lead. This research was true experimental research with a randomized post-test-only control group design using mice divided into 7 groups, namely, group K- (group was given a standard diet), acetate lead 40 mg/kgBW induced K+, P1 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 75 mg/kgBW, P2 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 150 mg/kgBW, P3 was induced by acetate lead 40 mg/kgBW and vitamin C 75 mg/kgBW, P4 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 75 mg/kgBW as well as vitamin C 35 mg/kgBW, P5 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 150 mg/kgBW as well as vitamin C 75 mg/kgBW for 30 days. On the 31st day, the mice were killed to check their catalase enzyme activity. In this research, there was a significant (P<0.05) increase in catalase enzyme activity between the K- with K + group and between the K+ group with P4. The conclusion was that jamblang leaves extract was able to increase catalase enzyme activity after being induced by lead acetate. Keywords: Lead acetate, jamblang leaf extract, vitamin C, catalase enzyme activity, rats.