Pyrolysis mechanism of isolated microalgal composition and their potential as liquid biofuels: Neutral lipids, phospholipids, glycolipids, and sterols

• Neutral lipids, phospholipids and glycolipids were isolated from microalgal lipids. • The main carbon number distribution in pyrolysis products were C 16 , C 18 and C 20 . • Long-chain carboxylic acids were the main composition after pyrolysis. • Glycosyl in glycolipids lead to the formation of some cyclic compounds. • Sterols are beneficial for the formation of polycyclic aromatic hydrocarbons. Microalgal lipids are the important feedstock of alternative liquid biofuels due to their similar structure to liquid hydrocarbons and high content in microalgae. However, complex composition of microalgal lipids hinders their practical application as biofuels and their reaction mechanism is also not fully studied, especially for phospholipids and glycolipids. In this study, the key fractions (neutral lipids, phospholipids, and glycolipids) in microalgal lipids were separated by a solid phase extraction method. There was a predominant content (39.5 %) for glycolipids in lipids of microalga Chlorella sp., while there was similar content between the neutral lipids, glycolipids, and phospholipids for microalga Nanochloropsis sp.. The pyrolysis characterization of each lipid fraction was analyzed via pyrolysis–gas chromatography–mass spectrometry. The main carbon number distribution in pyrolysis products were C 16 , C 18 , and C 20 for all lipid fractions. The main compound types were long-chain chemicals with the carbon number range of C 16 –C 20 , especially carboxylic acids. The pyrolysis mechanism was elucidated based on carbon number distribution and compound type distribution in pyrolysis products. The major decomposition reactions were mainly decarboxylation, decarbonylation, glycerol fragmentation, and steroid cracking to form hydrocarbons, acids, and esters. Due to the typical characteristics of sterols in microalgal lipids, the pyrolysis products presented more cyclic structures, especially aromatic hydrocarbons as a typical composition in liquid transportation fuels. This study provide necessary information for large-scale application of microalgal lipids in liquid biofuels via pyrolysis pathway, which is also benefit for the research of novel composite catalysts during catalytic pyrolysis to further upgrading of non-catalytic pyrolysis products.