Initial quality assessment and qualitative interpretation of protein film electrochemistry catalytic data
When a redox enzyme is wired to an electrode under conditions of direct electron transfer (DET), its activity can be simply detected as a current. This approach has been reviewed extensively, but here we address a gap in the literature by discussing the initial qualitative interpretation and assessment of catalytic DET electrochemical data. Topics addressed here include electroactive coverage, turnover frequencies, mass transport limitations, film loss, redox-driven (in)activation, signal corrections, distinction between steady-state and transient responses, and identification of non-ideal behaviors. Based on our group's expertise, we provide explanations, general advice, and prescriptive guidance to help readers understand experimental issues.
Authors
- Laura V. Opdam (ORCID: https://orcid.org/0000-0001-5099-1066)
- Christophe Léger (ORCID: https://orcid.org/0000-0002-8871-6059)
- Andrea Fasano (ORCID: https://orcid.org/0000-0002-8322-1620)
- Vincent Fourmond (ORCID: https://orcid.org/0000-0001-9837-6214)
- Anna Aldinio-Colbachini (ORCID: https://orcid.org/0009-0005-2585-8809)
- Miriam Malagnini (ORCID: https://orcid.org/0009-0008-0537-071X)
- Andrea di Giuliantonio (ORCID: https://orcid.org/0009-0002-6523-8987)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Aix-Marseille Université (FR)
Publication Details
- Journal
- Bioelectrochemistry
- Published
- 2025-03-08
- DOI
- https://doi.org/10.1016/j.bioelechem.2025.108967
- Citations
- 2
- Primary Topic
- Metalloenzymes and iron-sulfur proteins
- Type
- article
- Field-Weighted Citation Impact
- 0.73
Funders
- Aix-Marseille Université
- European Commission
- Agence Nationale de la Recherche
- Centre National de la Recherche Scientifique
- HORIZON EUROPE Marie Sklodowska-Curie Actions
- HORIZON EUROPE European Innovation Council