An atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions
Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate of RNA. Inversely, RNA riboregulates the interactions and functions of the associated proteins. Dysregulation of ribonucleoprotein functions is linked to diseases such as cancer and neurological disorders. In dividing cells, RNA and RNA-binding proteins are present in mitotic structures, but their impact on cell division remains unclear. By applying the proteome-wide R-DeeP strategy to cells synchronized in mitosis versus interphase integrated with the RBP2GO knowledge, we provided an atlas of RNA-dependent proteins in cell division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 and TPX2 as unconventional RNA-binding proteins. KIFC1 was identified as a new substrate of AURKA, and new TPX2-interacting protein. Their pair-wise interactions were RNA dependent. In addition, RNA stimulated AURKA kinase activity and stabilized its conformation. In this work, we highlighted riboregulation of major mitotic factors as an additional complexity level of cell division.
Authors
- Rainer Will (ORCID: https://orcid.org/0000-0001-6049-2690)
- Simona Cantarella (ORCID: https://orcid.org/0000-0002-2081-5966)
- Melina Klostermann (ORCID: https://orcid.org/0000-0003-3122-1095)
- Isha Nasa (ORCID: https://orcid.org/0000-0001-7699-795X)
- Dominic Helm (ORCID: https://orcid.org/0000-0001-9321-2069)
- Kathi Zarnack (ORCID: https://orcid.org/0000-0003-3527-3378)
- Jeanette Seiler
- Julian König (ORCID: https://orcid.org/0000-0002-9398-5421)
- Martin Schneider (ORCID: https://orcid.org/0000-0002-4128-4930)
- Maïwen Caudron‐Herger (ORCID: https://orcid.org/0000-0002-7030-8153)
- Sven Diederichs (ORCID: https://orcid.org/0000-0001-7901-4752)
- Arminja N. Kettenbach (ORCID: https://orcid.org/0000-0003-3979-4576)
- Varshni Rajagopal
- Bianca Kaifer
- Franziska Herget
- Jana Theiss
Institutions
- Dartmouth College (US)
- University of Freiburg (DE)
- German Cancer Research Center (DE)
- Cotton (United States) (US)
- Heidelberg University (DE)
- University of Würzburg (DE)
- University Medical Center Freiburg (DE)
- DKFZ-ZMBH Alliance (DE)
- Institute of Molecular Biology (DE)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-03-08
- DOI
- https://doi.org/10.1038/s41467-025-57671-3
- Citations
- 8
- Primary Topic
- RNA Research and Splicing
- Type
- article
- Field-Weighted Citation Impact
- 6.45
Funders
- Baden-Württemberg Stiftung
- Center for Strategic Scientific Initiatives, National Cancer Institute
- Deutsche Forschungsgemeinschaft
- Deutsche Krebshilfe
- National Institutes of Health