Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis
The declining birth rates and fertility challenges in Europe have intensified global concerns over rising infertility, particularly among women. This study decisively investigates follicular fluid-related extracellular vesicles (FF-EVs) from infertile patients with polycystic ovary syndrome (PCOS) or diminished ovarian reserve (DOR) undergoing in vitro fertilization (IVF), comparing them to a healthy control group. We have identified significant variations in protein content and polydispersity in crude follicular fluid using UV-Vis absorption and dynamic light scattering (DLS) techniques. Furthermore, the morphology of the extracellular vesicles (EVs) and the patterns of non-coding RNA content, including miRNAs, reveal distinct differences in infertile patients. These findings offer critical insights into the molecular signatures associated with these conditions. This study plays a vital role in advancing reproductive healthcare by pinpointing potential targets that can enhance diagnosis and deepen our understanding of ovarian disorders.
Authors
- Pascale Even‐Hernandez (ORCID: https://orcid.org/0000-0002-2187-1150)
- Claire Heichette
- Christophe Penno (ORCID: https://orcid.org/0000-0002-7698-5278)
- Grégory Moulin (ORCID: https://orcid.org/0000-0002-7529-474X)
- Célia Ravel (ORCID: https://orcid.org/0000-0001-5013-6932)
- Aurélien Dupont (ORCID: https://orcid.org/0000-0002-3947-0806)
- Valérie Marchi (ORCID: https://orcid.org/0000-0002-6565-7201)
- Solène Ducarre
- Regina Maria Chiechio (ORCID: https://orcid.org/0000-0002-6469-3059)
- Abdelhak El Amrani (ORCID: https://orcid.org/0000-0001-6078-8379)
- Ester Butera (ORCID: https://orcid.org/0000-0003-1484-3204)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- École des Hautes Études en Santé Publique (FR)
- University College Cork (IE)
- University of Catania (IT)
- Ecosystèmes, Biodiversité, Evolution (FR)
- Institut des Sciences Chimiques de Rennes (FR)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- APC Microbiome Institute (IE)
- Centre Hospitalier Universitaire de Rennes (FR)
- Université de Rennes (FR)
Publication Details
- Journal
- Journal of Extracellular Biology
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1002/jex2.70041
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81