A Rapid, Detection and Separation of Eugenol and Its Isomers Isolated From <i>Polianthes tuberosa</i> (Linn.) Flower by Supercritical Fluid Chromatography ‐ Photodiode Array‐Mass Spectrometry ‐ A Method Development, Validation, and Stability Studies

Supercritical fluid chromatography (SFC) is widely recommended as an alternative to normal-phase liquid chromatography in the emerging field of green analytical chemistry. SFC separations are quick, efficient, and environmentally friendly separation of chiral and achiral molecules. Due to this, SFC is economically feasible in separation science technology. The identification and separation of chiral compounds is crucial in understanding their pharmacological significance. Polianthes tuberosa (Linn.) is traditionally considered an ornamental and medicinal plant worldwide. For the separation of eugenol and its derivatives from the P. tuberosa flower, a rapid and highly efficient/SFC approach was developed and implemented in this study. However, no research into the SFC, method validation, and absolute measurement of eugenol has been published from the P. tuberosa flower. We used SFC coupled with mass spectrometry (SFC-MS) to develop a quick and effective approach for characterizing and quantifying eugenol in this investigation. Further, molecular docking studies and molecular dynamic simulations (MDSs) have been performed for the potent isomer of eugenol. This research has uncovered important insights of SFC in drug discovery. Additionally, molecular docking and molecular dynamics simulation studies have provided clarity on how the compound interacts with different biological targets.