Cryo-EM structure of the <i>Pseudomonas aeruginosa</i> MexY multidrug efflux pump
Here, we report the cryo-electron microscopy structure of the MexY multidrug efflux pump, posing the possibility that this pump is capable of capturing antibiotics from both the central cavity and the periplasmic cleft of the pump. The results indicate that MexY may utilize charged residues to bind and export drugs, mediating resistance to these antibiotics.
Authors
- James E. Kirby (ORCID: https://orcid.org/0000-0003-0392-4500)
- Nishanth Sastry (ORCID: https://orcid.org/0000-0002-4053-0386)
- Rakesh Maharjan (ORCID: https://orcid.org/0000-0003-2217-3191)
- Lucius Chiaraviglio (ORCID: https://orcid.org/0000-0002-2613-6614)
- Zhemin Zhang (ORCID: https://orcid.org/0000-0003-0135-4970)
- Meng Cui (ORCID: https://orcid.org/0000-0002-3895-135X)
- Eunsun Yu (ORCID: https://orcid.org/0000-0001-5912-1227)
- William D. Gregor (ORCID: https://orcid.org/0009-0002-7722-614X)
Institutions
- Northeastern University (US)
- Beth Israel Deaconess Medical Center (US)
- Harvard University (US)
- Hadassah Medical Center (IL)
- University School (US)
- Case Western Reserve University (US)
Publication Details
- Journal
- mBio
- Published
- 2025-03-05
- DOI
- https://doi.org/10.1128/mbio.03826-24
- Citations
- 7
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 4.14