IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL

This study aims to identify DNA barcodes with the rbcL gene locus and reconstruct the phylogenetic relationship of the Balakka plant with species belonging to the genus Phyllanthus. This research includes experimental research by isolating DNA, amplification DNA using rbcL gene as primer, electrophoresis and sequencing. DNA was isolated with a commercial kit, amplification was carried out using the Polymerase Chain Reaction method. Sequence results and phylogenetic tree reconstruction were analyzed by Bioinformatics (MEGA 11). The results showed that the number of amplicons from the consensus of the two primers on the balakka plant sample was 557 bp. The phylogenetic tree of the Balakka sequence formed a sister clade with Phyllantus emblica KY9888320.1 with 66% similarity, and P. emblica grouped together, but still had common ancestor branches with other species of the Phyllantus genus using the Neighbor-Joining method, and Kimura 2-parameter calculations.