Optimization of Self Nanoemulsifying Drug Delivery System of Mature Green Betel Leaf Fraction with D-Optimal Method and Antifungal Activity of Candida albicans

The purpose of the study was to see the most active fraction in providing activity, the influence and optimal proportion of oleic acid, tween 80, and propylene glycol on characterization and the optimal formula that has increased activity. The Fractionation Process uses water, ethyl acetate, and n-hexane as solvents. Activity tests use concentration variations of 0.4%, 0.8%, 1.6%. SNEDDS formulation uses 15% -20% oleic acid, 55% -60% tween 80, and 20% -25% propylene glycol and is tested for characterization and optimization using D-optimal. The optimum formula was tested for antifungal activity. The results of the antifungal activity test showed that the ethyl acetate fraction had the most active activity. Characterization showed that an increase in oleic acid provided an increase in emulsification time, particle size, polydispersion index, zeta potential and a decrease in drug loading. The increase of tween 80 and propylene glycol resulted in a decrease in emulsification time, particle size, polydispersity index, zeta potential, and an increase in drug loading. The optimum formula at the ratio of oleic acid: tween 80:propylene glycol = 16.1:58.9:25 with an emulsification time of 70 seconds, particle size of 186.98, polydispersity index of 0.331, zeta potential of -32.02, and drug loading of 74.3%. The optimum formula resulted in an increase in DDH of 1.3 mm.