F5 6665A>G Polymorphism Is Associated with Increased Risk of Venous Thromboembolism in Females

The main goal of our study was to assess the potential effect of the polymorphism of the coagulation-related genes <i>F2</i>, <i>F5</i>, and <i>F13A</i> on the risk of venous thromboembolism (VTE) development. The study was conducted at the Clinical Center, Podgorica, Montenegro, and included 103 VTE patients and 106 sex- and age-matched healthy controls. Demographic, clinical, and laboratory data were obtained from the medical records and questionnaires. Genotyping for <i>F2</i> 19911A>G (rs3136516), <i>F5</i> 6665A>G (rs6027), and <i>F13A</i> 102G>T (rs5985) was performed by allele-specific PCR. Controlling for the effect of known risk factors, the presence of at least one variant <i>F5</i> 6665 G allele conferred a significantly higher risk of VTE among females [OR (95%CI): 64.06 (5.38; 763.61)], but not among males. In addition, thromboembolic events were associated with comorbidities [OR (95%CI): 197.10 (19.17; 2026.19)], overweight [OR (95%CI): 33.59 (2.47; 456.65)], and the presence of <i>F2</i> 20210G>A [OR (95%CI): 32.43 (4.21; 249.77)] and <i>F5</i> 1601G>A [OR (95%CI): 144.80 (13.59; 1542.63)] in females, as well as with comorbidities [OR (95%CI): 6.32 (1.90; 20.98)], family history of VTE [OR (95%CI): 8.10 (2.28; 28.83)], and the presence of <i>F5</i> 1601G>A [OR (95%CI): 20.10 (2.34; 173.02)] in males. Our study reports an association between the presence of at least one <i>F5</i> 6665G variant allele and an increased risk of VTE development in females. Our results indicate that <i>F5</i> 6665A>G, in combination with other confirmed factors of influence, such as comorbidities, overweight, <i>F2</i> 20210G>A, and <i>F5</i> 1601G>A, could contribute to VTE risk prediction in females.