In situ 3D bioprinted GDMA/Prussian blue nanozyme hydrogel with wet adhesion promotes macrophage phenotype modulation and intestinal defect repair

Developing hydrogels with wet-adhesion, immunomodulation and regenerative repair capabilities in intestinal repair remains a formidable challenge. In the present study, the development of an anti-inflammatory, wet-adhesive, decellularized extracellular matrix hydrogel produced using three-dimensional (3D) -printing technology is presented. This hydrogel, which integrates gelatin and dopamine, was demonstrated to display excellent wet-adhesion properties, fully harnessing the outstanding regenerative potential of the decellularized small-intestine matrix. Furthermore, the integration of Prussian Blue nanozymes imparted significant anti-inflammatory and antioxidant properties. Through modulating macrophage polarization, the hydrogel was not only found to enhance tissue repair, but also to substantially mitigate inflammation. <i>In vivo</i> experiments (namely, histopathological analyses using a rat model) demonstrated that this hydrogel was able to effectively enhance tissue regeneration and healing in models of intestinal damage. In conclusion, through the utilization of 3D-printing technology, the present study has shown that the precise manufacturing and customization of the hydrogel to various shapes and sizes of intestinal defects may be executed, thereby providing an innovative strategy for intestinal repair. This advanced hydrogel has therefore been shown to hold significant promise as a bioadhesive for both emergency repair and regenerative therapy.