Protocol to purify and culture human pancreatic cancer cells from patient-derived xenografts
Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive inter- and intratumoral heterogeneity, along with a significant stromal component. This protocol outlines steps to generate patient-derived xenografts (PDXs), isolate and enrich human PDAC epithelial cells, and verify their identity using droplet digital PCR (ddPCR) and human-specific markers. It provides a robust approach for culturing cancer epithelial cells to develop a human PDAC model system. For complete details on the use and execution of this protocol, please refer to Grygoryev et al.<sup>1</sup>.
Authors
- Terry K. Morgan (ORCID: https://orcid.org/0000-0003-0742-5012)
- Jason M. Link (ORCID: https://orcid.org/0000-0002-6892-0431)
- Brett C. Sheppard (ORCID: https://orcid.org/0000-0002-2182-7547)
- Dove Keith
- Julien Tessier
- Brittany L. Allen-Petersen (ORCID: https://orcid.org/0000-0002-7436-9412)
- Dmytro Grygoryev
- Taelor Ekstrom (ORCID: https://orcid.org/0000-0001-9829-9570)
- Rosalie C. Sears (ORCID: https://orcid.org/0000-0003-1558-2413)
- Elise C. Manalo (ORCID: https://orcid.org/0000-0003-2752-5280)
- Seung-Won Lee (ORCID: https://orcid.org/0000-0001-8893-8332)
- Jung-Sun Kim (ORCID: https://orcid.org/0000-0003-2454-8065)
- Mark Berry (ORCID: https://orcid.org/0000-0002-8534-2012)
- Marilynn Chow-Castro
Institutions
- Oregon Health & Science University (US)
- Oregon Medical Research Center (US)
- Pancreatic Cancer UK (GB)
- University of Portland (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1016/j.xpro.2025.103672
- Citations
- 2
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 2.09