Evaluating the Cryoprotective Effects of Butylated Hydroxytoluene on Semen Quality Parameters of Phasianus colchicus

Cryopreservation is critical for the preservation of genetic resources and the propagation of endangered species. The choice of proper cryoprotectants significantly affects the success of cryopreservation. The current study investigated the impact of butylated-hydroxytoluene (BHT) on sperm microscopic assays in Ring-necked Pheasants at various post-processing phases, including post-dilution, post-cooling, post-equilibration, and post-thawing. Semen samples were collected and cryopreserved at various times with BHT (0.0 mM (control), 0.5 mM, 1.0 mM, 1.5 mM, and 2.0 mM. Acrosomal integrity (AI), viability, plasma membrane integrity (PMI), and sperm motility were all assessed as significant markers of sperm quality. It was discovered that using a 1.0 mM concentration of BHT had a significant (p > 0.05) influence on each stage of cryopreservation, resulting in improvements in sperm motility, PMI, viability, and AI when analyzed in both control and other groups. These findings emphasize BHT's cryoprotective qualities of BHT in protecting the quality and integrity of Ring-necked Pheasant sperm during cryopreservation. Further research is needed to understand the mechanisms by which BHT exerts its protective effects and to maximize its use in assisted reproductive treatments. The use of BHT as a cryoprotectant has the potential to aid in the conservation of endangered bird species, such as the Ring-necked Pheasant.