The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to Meloidogyne incognita

Long non-coding RNAs (lncRNAs) represent a class of non-coding RNAs. In the study of <i>Pseudomonas putida</i> Sneb821-induced tomato resistance to <i>Meloidogyne incognita</i>, reverse transcription polymerase chain reaction (RT-PCR) was employed to validate 12 lncRNAs in tomato. Among them, the lncRNA47258/miR319b/TCP molecular regulatory module was likely implicated in the process of Sneb821-induced tomato resistance against <i>M. incognita</i>. Through the application of tomato hairy root and virus-induced gene silencing (VIGS) technologies for the investigation of lncRNA47258, it was determined that lncRNA47258 could target the <i>TCP</i> (<i>Solyc07g062681.1</i>) gene and modulate the metabolic pathway of tomato jasmonic acid-related indices, thereby impeding the infection of <i>M. incognita</i>. Moreover, the overexpression of the target gene <i>TCP</i> (<i>Solyc07g062681.1</i>) using tomato hairy root technology demonstrated that it could regulate the jasmonic acid synthesis pathway in tomato, consequently obstructing the infection and suppressing the development of <i>M. incognita</i>. Collectively, lncRNA47258/miR319b/<i>TCP</i> (<i>Solyc07g062681.1</i>) was preliminarily verified to be involved in the Sneb821-induced resistance process against <i>M. incognita</i> in tomato.