15P Topical strategy to prevent skin toxicities from bispecific-EGFR inhibitors by blocking drug-receptor interactions in skin

Results: Through the highly efficient isolation and immortalization of tumor-or TLSderived B cells, our approach captures the B cell diversity and all Ig-isotypes from all tissues.As opposed to the labor intensive and costly approach of ex vivo BCR sequencing and subsequent recombinant antibody production and validation, our B cell Tumor Libraries (TuLibs) secrete antibodies into the culture supernatant while retaining indefinite expansion capabilities, allowing for immediate functional screening of antibodies and rapid identification of unique tumor-specific B cell clones.Moreover, our immortalized B cells retain the ability to undergo somatic-hypermutation, allowing for in vitro affinity maturation of immortalized B cell clones against targets of interest.Conclusions: Altogether, Kling Biotherapeutic's proprietary platform allows the comprehensive functional screening and affinity maturation of human and animal B cell repertoires from multiple tissues sources.TuLibs represents a significant advancement in our ability to identify new immuno-functional targets and antibodies for biopharmaceutical development of tumor targeted therapies.