Alternative Splicing Analysis Reveals Adrenergic Signaling as a Novel Target for Protein Arginine Methyltransferase 5 (PRMT5) in the Heart

Adrenergic signaling is critical for maintaining cardiac function and works by regulating heart rate, contractility, and stress responses. Protein arginine methyltransferase 5 (PRMT5), a key enzyme involved in gene expression, signal transduction, and RNA processing, has been revealed to be an important factor in heart disease. However, its specific effects on adrenergic signaling have not been fully elucidated. In this study, we examined the role of PRMT5 in the heart by analyzing alternative splicing events in cardiac tissues from <i>Prmt5</i>-deficient mice. High-throughput RNA sequencing and bioinformatics analyses identified significant alterations in alternative splicing, particularly in genes related to adrenergic signaling, which were further validated using reverse transcription PCR. These results underscore the role of PRMT5 as an important regulator of alternative splicing in the heart and identify adrenergic signaling as a novel target. Collectively, our findings offer new insights into the molecular mechanisms underlying cardiac function and suggest that PRMT5 is a potential therapeutic target for heart diseases.