Sequencing Analysis of Lactic Acid Bacteria as Probiotics from Ettawah Goat's Milk as Antimicrobial

The utilization of fermented milk as probiotics to address nutritional concerns in Indonesia has been limited. Multiple studies have demonstrated that probiotics can mitigate dysbiosis by altering the gut microbiota composition. One reason is milk utilization from domesticated animals, attributed to the nutritional composition and presence of lactic acid bacteria (LAB) as probiotics in goat milk. This work aimed to evaluate the molecular potential of probiotic isolates derived from goat milk utilizing the 16S rRNA gene and their antimicrobial activities. This study employed the sequencing analysis of potential probiotic isolates from goat milk using the 16S rRNA gene. The analysis focused on examining the arrangement of lactic acid bacteria DNA in terms of acid-base length and evaluating their antimicrobial activity against Escherichia coli, Salmonella sp., Klebsiella sp., as well as their antifungal activity against Candida albicans. The research findings were obtained by analyzing the amplification of DNA sequences from probiotic isolates found in goat milk, namely in a 1391 bp region. Furthermore, the antibacterial activity was assessed by measuring the diameter of the clear Zone formed on the test bacteria E. coli (7.10 mm), Salmonella sp. (6.10 mm), Klebsiella sp. (9.13 mm), and the fungus Candida albicans (15.19 mm). This study proves that LAB isolates derived from goat milk possess probiotic properties. This is supported by their amplification of the 16S rRNA gene, sequencing analysis showing similarity to Lactiplantibacillus plantarum, and their ability to inhibit the growth of pathogenic bacteria and exhibit antifungal activity.