Separating Absorption and Scattering Coefficients for the Characterization of Optical Turbid Media Using Structured Laser Illumination Planar Imaging and Conventional Spectroscopy Technique

We report on the potential of combining the Structured Laser Illumination Planar Imaging (SLIPI) technique and optical spectroscopy for characterizing scattering and absorbing liquids. Water solutions of milk and coffee were investigated at 10 different turbidity levels. The SLIPI technique eliminates the light intensity contributions from multiply scattered photons in these solutions, enabling accurate and reliable measurements of their extinction coefficients . Optical spectroscopy, on the other hand, provides complementary insights into the interaction between light and matter, allowing precise spectral analysis. The linearity of the extinction coefficient measurements obtained using the SLIPI technique as a function of concentration, combined with spectroscopic approximations, enables the separation of absorption and scattering coefficients. This opens the possibility for precise characterization of both absorption and scattering properties in dense liquid media.