Cas9-expressing cattle using the PiggyBac transposon all-in-one system
These data demonstrate, for the first time, that Cas9-expressing cattle were successfully born, and this transgene was transmitted to the next-generation calves (F1) and F2 embryos. In addition, somatic and germ cells derived from F0 and F1generations were used to evaluate the potential for gene editing (knockout and knock-in) in multiple genes. PRNP-mutated F1 cattle are currently being raised as a resistance model for bovine spongiform encephalopathy. These transgenic bovine models and their derivatives will serve as a valuable resource for both in vitro and in vivo genome editing, advancing our genetic understanding of bovine genomics and diseases.
Authors
- Kyeong-Hyeon Eom
- Gyeong‐Min Gim (ORCID: https://orcid.org/0000-0002-9352-094X)
- Goo Jang (ORCID: https://orcid.org/0000-0003-3804-1871)
- Hyeong-Jong Kim
- Won-You Lee
- Woojae Choi (ORCID: https://orcid.org/0000-0002-0779-9236)
- Soo‐Young Yum (ORCID: https://orcid.org/0000-0002-9922-9168)
- Byeongho Moon (ORCID: https://orcid.org/0000-0003-1918-6709)
- Dae‐Jin Jung
- Jun Koo Yi (ORCID: https://orcid.org/0000-0003-2593-6529)
- Dae‐Hyun Kim (ORCID: https://orcid.org/0000-0002-3525-3812)
- Ji‐Hyun Lee (ORCID: https://orcid.org/0000-0003-2817-8006)
- Do Yoon Kim (ORCID: https://orcid.org/0000-0002-9934-5484)
- Dong-Hyeok Kwon (ORCID: https://orcid.org/0000-0003-1604-7877)
- Hee-Soo Kim
- Sang-Eun Han
- Woo-Sung Lee
- Song-Jeon Lee
Institutions
- Chonnam National University (KR)
- Hankyong National University (KR)
- Seoul National University (KR)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-03-04
- DOI
- https://doi.org/10.1186/s12864-025-11381-8
- Citations
- 2
- Primary Topic
- Animal Genetics and Reproduction
- Type
- article
- Field-Weighted Citation Impact
- 3.63