Genotyping Detection of Human Papillomavirus in Benign Prostate Hyperplasia in Ramadi City

Background: The presence of Human Papillomavirus (HPV) in Benign Prostate Hyperplasia (BPH) could suggest an important role of viral infection in the pathogenesis of BPH, potentially influencing treatment strategies. If a correlation is established, it could lead to increased awareness and screening for HPV in male populations, similar to cervical cancer screening in women. Objectives: To detect HPV serotypes in patients with BPH and to disclose their potential roles in the pathophysiology of BPH. Patients and methods: A case control study, involve collection of 75 specimens from male individuals with BPH and compared to 75 specimens from age-matched healthy male volunteers. The samples were processed at College of Medicine laboratories- University of Anbar and Private Specialized laboratories in Ramadi city from the period December 2023 to April 2024, patient samples were collected based on physicians' recommendations, suspecting a viral infection with human Papillomavirus. In both groups, a quantitative test was conducted for specific HPV IgM16 and HPV IgM18 in serum using an Enzyme Linked Immunosorbant Assay (ELISA), Genotyping of HPV in the urine were detected by conventional PCR technique. Results: The statistic results indicated that the mean value of specific HPV16 IgM-is 11.02±0.968 compared to control group value 0.647±0.132 at significant differences P= 0.0001, while mean value of –specific HPV18 IgM is 0.752±0.235 compared to control group value 0.165±0.015 at significant differences P=0.019. The molecular screening results indicated that the HPV16 serotype is most frequent in patients with BPH than HPV18, (78.7%) compared (20%)respectively. Conclusion: HPVs, mainly serotype 16 and 18 were detected in patients with BPH, the study also shows human immune response to HPV 16 and HPV 18 in men with BPH which provide valuable information about immune state, understand how BPH develop, find new ways for diagnoses and development of BPH treatments. Urine sampling appears to be a suitable surrogate sample for DNA HPV serotype testing in male with limited access to healthcare.