IN VITRO AND IN OVO COMPARATIVE TOXICOLOGICAL ASSESSMENT OF SELF-ASSEMBLING PEPTIDE-BASED DENTAL REPAIR AGENTS

A significant health issue is that dental demineralisation can cause dental caries and other systemic health concerns, negatively impacting overall well-being.Due to the developmental stages of enamel and dentin in children, they are particularly vulnerable.This study aimed to evaluate the safety of two self-assembling peptide-based gels (SAPs) used for dentin remineralisation.For the evaluation of SAPs', they were tested in vitro on human gingival fibroblasts (HGF) and in ovo on the chorioallantoic membrane of hen's eggs.Viability, morphology and nuclear integrity were evaluated in vitro, while irritation potential was determined in ovo.As a result of the findings, SAPs do not present a significant degree of cytotoxicity, providing minimal irritation and a protective, anti-irritating effect.Therefore, SAP-based gels suggest a biocompatible, safe and effective method for remineralisation, with low cytotoxicity and minimal irritation risk. RezumatO problem major de sntate, demineralizarea dentar, poate provoca carii dentare, precum i alte probleme sistemice de sntate, cu impact negativ asupra bunstrii generale.Datorit stadiilor de dezvoltare ale smalului i dentinei la copii, acetia sunt vulnerabili.Scopul studiului a fost evaluarea siguranei a dou geluri pe baz de peptide cu auto-asamblare (SAP) utilizate pentru remineralizarea dentinei.Pentru evaluarea SAP-urilor, acestea au fost testate in vitro pe fibroblaste gingivale umane (HGF) i in ovo pe membrana corioalantoidian a oulor de gin.Viabilitatea, morfologia i integritatea nuclear au fost evaluate in vitro, iar potenialul de iritare a fost determinat in ovo.Conform constatatrilor, SAP-urile nu prezint un grad semnificativ de citotoxicitate, avnd potenial iritativ minim i un efect protector, anti-iritant.Astfel, gelurile pe baz de SAP sugereaz o metod biocompatibil, sigur i eficient de remineralizare, cu citotoxicitate sczut i risc minim de iritare.