EVALUATION OF ANTI-MELANOMA, ANTIBACTERIAL AND ANTIOXIDANT EFFECTS OF A PHYTOCHEMICALLY CHARACTERIZED WATER-SOLUBLE FRACTION FROM GREEN COFFEE SEEDS

The green seeds of Coffea arabica are appreciated for the promotion of health and wellness.The current research focuses on the phytochemicals and bioactivity of the water-soluble fraction prepared from green coffee seeds.The first steps were to characterize the extract with the aid of HPLC, to record the FT-IR spectrum and to evaluate the content in total polyphenols.Subsequently, the antioxidant and antibacterial effects were determined.The last part of the research concerns the effect of the extract on the melanoma cell line A375.The water-soluble fraction, rich in chlorogenic acid, displayed a modest antibacterial effect, stronger against the fungus Candida parapsilosis than against the tested bacteria.It reduced the viability of A375 cells in a concentration-dependent manner, decreased cell number and confluence, presented a negative impact on the cell membrane integrity, and induced apoptosis.These effects warrant further investigations into the potential of the water-soluble fraction in the nutraceutical and dermatologic fields. RezumatSeminele netorefiate de Coffea arabica sunt apreciate pentru meninerea strii de sntate generale.Prezenta cercetare se concentreaz pe compoziia fitochimic i bioactivitatea fraciei hidrosolubile din aceste semine.Primii pai au fost reprezentai de caracterizarea extractului cu ajutorul HPLC, nregistrarea spectrului FT-IR i evaluarea coninutului n polifenoli totali.Ulterior, au fost determinate efectele antioxidante i antibacteriene.Ultima parte a cercetrii se refer la efectul extractului asupra liniei celulare de melanom A375.Fracia solubil n ap, bogat n acid clorogenic, a prezentat un efect antibacterian modest, mai intens contra ciupercii patogene Candida parapsilosis dect mpotriva bacteriilor testate.Extractul testat a redus viabilitatea celulelor A375 ntr-o manier dependent de concentraie, a sczut numrul de celule i confluena lor, a avut un impact negativ asupra integritii membranei celulare i a indus apoptoza.